2021/05/15 更新

写真a

タキハラ ハヤト
瀧原 速仁
TAKIHARA Hayato
所属
教育研究院 医歯学系 特任助教
医歯学総合研究科 特任助教
職名
特任助教
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外部リンク

学位

  • 博士 (生物資源科学) ( 2015年3月   日本大学 )

研究キーワード

  • 微生物生態学

  • バイオインフォマティクス

  • メタゲノム

研究分野

  • ライフサイエンス / システムゲノム科学  / バイオインフォマティクス

  • ライフサイエンス / 応用微生物学  / メタゲノム

経歴(researchmap)

  • 新潟大学大学院 医歯学総合研究科   バイオインフォーマティクス分野   特任助教

    2020年11月 - 現在

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    国名:日本国

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  • 新潟大学   大学院医歯学総合研究科   博士研究員

    2015年7月 - 2020年10月

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経歴

  • 新潟大学   医歯学総合研究科   特任助教

    2020年11月 - 現在

学歴

  • 日本大学   大学院 生物資源科学研究科   応用生命科学専攻

    2009年4月 - 2011年3月

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  • 日本大学   生物資源科学部   応用生物科学科

    2005年4月 - 2009年3月

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所属学協会

  • 日本微生物生態学会

    2009年

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論文

  • Higher genome mutation rates of Beijing lineage of Mycobacterium tuberculosis during human infection. 国際誌

    Mariko Hakamata, Hayato Takihara, Tomotada Iwamoto, Aki Tamaru, Atsushi Hashimoto, Takahiro Tanaka, Shaban A Kaboso, Gebremichal Gebretsadik, Aleksandr Ilinov, Akira Yokoyama, Yuriko Ozeki, Akihito Nishiyama, Yoshitaka Tateishi, Hiroshi Moro, Toshiaki Kikuchi, Shujiro Okuda, Sohkichi Matsumoto

    Scientific reports10 ( 1 ) 17997 - 17997   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mycobacterium tuberculosis (Mtb) strains of Beijing lineage have caused great concern because of their rapid emergence of drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses and the appearance of drug resistance have not been elucidated in human-infected Beijing strains. We tracked and obtained an original Mtb isolate of Beijing lineage from the 1999 tuberculosis outbreak in Japan, as well as five other isolates that spread in humans, and two isolates from the patient caused recurrence. Three isolates were from patients who developed TB within one year after infection (rapid-progressor, RP), and the other three isolates were from those who developed TB more than one year after infection (slow-progressor, SP). We sequenced genomes of these isolates and analyzed the propensity and rate of genomic mutations. Generation time versus mutation rate curves were significantly higher for RP. The ratio of oxidative versus non-oxidation damages induced mutations was higher in SP than RP, suggesting that persistent Mtb are exposed to oxidative stress in the latent state. Our data thus demonstrates that higher mutation rates of Mtb Beijing strains during human infection is likely to account for the higher adaptability and an emergence ratio of drug resistance.

    DOI: 10.1038/s41598-020-75028-2

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  • Innate Immune Responses in Serum and Cerebrospinal Fluid From Neonates and Infants Infected With Parechovirus-A3 or Enteroviruses. 国際誌

    Rie Habuka, Yuta Aizawa, Ryohei Izumita, Hisanori Domon, Yutaka Terao, Hayato Takihara, Shujiro Okuda, Akihiko Saitoh

    The Journal of infectious diseases222 ( 4 ) 681 - 689   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Parechovirus (PeV)-A3 and enteroviruses (EV) are the most common viruses causing sepsis and meningoencephalitis in neonates and young infants. Clinical manifestations of PeV-A3 infection are more severe than those of EV infection, and no pleocytosis with a positive polymerase chain reaction (PCR) result for PeV-A3 in cerebrospinal fluid (CSF) are characteristic findings. We hypothesized that innate immune responses to PeV-A3 and EV are distinct in serum and CSF. METHODS: We evaluated 22 cytokines/chemokines in serum and CSF from PeV-A3- or EV-infected patients younger than 4 months in Niigata, Japan, from 2015 through 2018. Infection was diagnosed with real-time PCR followed by sequencing. Febrile neonates and infants with sepsis-like syndrome who had negative bacterial culture and viral PCR for both PeV-A and EV were also included (non-PeV-A/EV patients). RESULTS: Among 192 febrile patients, we evaluated 16 PeV-A3-infected, 15 EV-infected, and 8 non-PeV-A/EV patients. Serum pro-/anti-inflammatory cytokine/chemokine levels were higher in PeV-A3-infected patients than in EV-infected patients (P < .02). Although most cytokine/chemokine were elevated in CSF from EV-infected patients, levels were low or undetectable in PeV-A3-infected and non-PeV-A/EV patients (P < .001). CONCLUSIONS: Distinct cytokine/chemokine patterns in serum and CSF may explain the different clinical manifestations of PeV-A3-infected and EV-infected neonates and young infants.

    DOI: 10.1093/infdis/jiaa131

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  • Rice Endosperm Protein Administration to Juvenile Mice Regulates Gut Microbiota and Suppresses the Development of High-Fat Diet-Induced Obesity and Related Disorders in Adulthood. 国際誌

    Yuki Higuchi, Michihiro Hosojima, Hideyuki Kabasawa, Shoji Kuwahara, Sawako Goto, Koji Toba, Ryohei Kaseda, Takahiro Tanaka, Nobutaka Kitamura, Hayato Takihara, Shujiro Okuda, Masayuki Taniguchi, Hitoshi Arao, Ichiei Narita, Akihiko Saito

    Nutrients11 ( 12 )   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Obesity and related disorders, which are increasing in adults worldwide, are closely linked to childhood diet and are associated with chronic inflammation. Rice endosperm protein (REP) intake during adulthood has been reported to improve lipid metabolism and suppress the progression of diabetic kidney disease in animal models. However, the effects of REP intake during childhood on adulthood health are unclear. Therefore, we used a mouse model to experimentally investigate the preconditioning effects of REP intake during childhood on the development of obesity and related disorders in adulthood. Male C57BL/6J mice were pair-fed a normal-fat diet containing casein or REP during the juvenile period and then a high-fat diet (HFD) containing casein or REP during adulthood. Mice fed REP during the juvenile period showed better body weight, blood pressure, serum lipid profiles, lipopolysaccharide (LPS)-binding protein levels, and glucose tolerance in adulthood than those fed casein during the juvenile period. HFD-induced renal tubulo-glomerular alterations and hepatic microvesicular steatosis were less evident in REP-fed mice than in casein-fed ones. REP intake during the juvenile period improved HFD-induced dysbiosis (i.e., Escherichia genus proliferation and reduced gut microbiota diversity), thereby suppressing endotoxin-related chronic inflammation. Indeed, REP-derived peptides showed antibacterial activity against Escherichia coli, a major producer of LPS. In conclusion, REP supplementation during the juvenile period may regulate the gut microbiota and thus suppress the development of obesity and related disorders in adulthood in mice.

    DOI: 10.3390/nu11122919

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  • Development of a Simple Nonbiological Method for Converting Lignin-Derived Aromatics into Nonaromatic Polymeric Substances with Fluorescent Activity

    Noriyuki Iwabuchi, Yuki Sakano, Hajime Takiguchi, Hayato Takihara, Michio Sunairi, Hiroshi Matsufuji

    ACS SUSTAINABLE CHEMISTRY & ENGINEERING4 ( 8 ) 4411 - 4416   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    We recently reported that Pseudomonas sp. ITH-SA-1 can transform the lignin-derived aromatic compound syringaldehyde (SYAL) into nonaromatic polymeric substances with fluorescent activity (NAPSFA). NAPSFA are particularly rare organic substances that fluoresce despite the absence of aromatic rings. In this study, we developed a simple method for producing NAPSFA using a nonbiological process. Incubation of the SYAL metabolite 3-O-methyl gallate (3-MGA) produced by Pseudomonas sp. ITH-SA-1 with Marine Broth (MB) or Luria-Bertani broth (LB) produced fluorescence even in the absence of bacteria, suggesting that fluorescent substances were produced from 3-MGA by a nonbiological process. 3-MGA reacted with tryptone and peptone, which are the primary nitrogen and phosphate sources in MB and LB, to produce the fluorescent substances. The fluorescent substances produced by the reaction of 3-MGA with tryptone exhibited excitation and emission peaks at 370 and 505 nm, respectively, which are nearly identical to those of NAPSFA (365/498 nm). The average molecular weight of the fluorescent substances (4.2 kDa) was lower than that of NAPSFA (7.2 kDa). ATR-FTIR and NMR analyses revealed that the molecules contained no aromatic rings, similar to NAPSFA. Our results demonstrate that nonaromatic fluorescent substances can be synthesized via a simple chemical reaction.

    DOI: 10.1021/acssuschemeng.6b01009

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  • Mg(2+)-Dependent Control of the Spatial Arrangement of Rhodococcus erythropolis PR4 Cells in Aqueous-Alkane Two Phase Culture Containing n-Dodecane.

    Hayato Takihara, Yumiko Akase, Michio Sunairi, Noriyuki Iwabuchi

    Microbes and environments31 ( 2 ) 178 - 81   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We recently reported that a close relationship exists between alkane carbon-chain length, cell growth, and translocation frequency in Rhodococcus. In the present study, we examined the regulation of the spatial arrangement of cells in aqueous-alkane two phase cultures. An analysis of the effects of minerals on cell localization revealed that changes in the concentration of MgSO4 in two phase cultures containing n-dodecane (C12) altered cell localization from translocation to adhesion and vice versa. Our results indicate that the spatial arrangement of cells in two phase culture systems is controlled through the regulation of MgSO4 concentrations.

    DOI: 10.1264/jsme2.ME15196

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  • Transformation of Lignin-Derived Aromatics into Nonaromatic Polymeric Substances with Fluorescent Activities (NAPSFA) by Pseudomonas sp ITH-SA-1

    Noriyuki Iwabuchi, Hajime Takiguchi, Takashi Hamaguchi, Hayato Takihara, Michio Sunairi, Hiroshi Matsufuji

    ACS Sustainable Chemistry & Engineering3 ( 11 ) 2678 - 2685   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    We examined bacteria capable of transforming lignin-derived compounds into industrially or economically valuable substances from the seawater after the Great East Japan Earthquake-caused tsunami. Pseudomonas sp. ITH-SA-1 produced water-soluble fluorescent substances from the lignin-derived aromatic, syringaldehyde (SYAL). They: are polymeric substances derived from 3-O-methyl gallate produced through transformation of SYAL via syringate; are not known compounds reported previously; have excitation/emission peaks at 365/498 nm, respectively; and have an average molecular weight of 7.2 kDa. Despite the fact that aromatic structure generally plays an important role in the planarity and rigidity of organic fluorescent substance, the spectroscopic analyses revealed that aromatic rings were not detected in the molecules. Their activity is particularly rare and the biotransforming capabilities will contribute to the development of new basic techniques for the effective utilization of lignin.

    DOI: 10.1021/acssuschemeng.5b00503

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  • Enhanced translocation and growth of Rhodococcus erythropolis PR4 in the alkane phase of aqueous-alkane two phase cultures were mediated by GroEL2 overexpression.

    Hayato Takihara, Jun Ogihara, Takao Yoshida, Shujiro Okuda, Mutsuyasu Nakajima, Noriyuki Iwabuchi, Michio Sunairi

    Microbes and environments29 ( 4 ) 346 - 52   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously reported that R. erythropolis PR4 translocated from the aqueous to the alkane phase, and then grew in two phase cultures to which long-chain alkanes had been added. This was considered to be beneficial for bioremediation. In the present study, we investigated the proteins involved in the translocation of R. erythropolis PR4. The results of our proteogenomic analysis suggested that GroEL2 was upregulated more in cells that translocated inside of the pristane (C19) phase than in those located at the aqueous-alkane interface attached to the n-dodecane (C12) surface. PR4 (pK4-EL2-1) and PR4 (pK4-ΔEL2-1) strains were constructed to confirm the effects of the upregulation of GroEL2 in translocated cells. The expression of GroEL2 in PR4 (pK4-EL2-1) was 15.5-fold higher than that in PR4 (pK4-ΔEL2-1) in two phase cultures containing C12. The growth and cell surface lipophilicity of PR4 were enhanced by the introduction of pK4-EL2-1. These results suggested that the plasmid overexpression of groEL2 in PR4 (pK4-EL2-1) led to changes in cell localization, enhanced growth, and increased cell surface lipophilicity. Thus, we concluded that the overexpression of GroEL2 may play an important role in increasing the organic solvent tolerance of R. erythropolis PR4 in aqueous-alkane two phase cultures.

    DOI: 10.1264/jsme2.ME13158

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  • Rhodococcus rhodochrous ATCC12674 becomes alkane-tolerant upon GroEL2 overexpression and survives in the n-octane phase in two phase culture.

    Hayato Takihara, Chiaki Matsuura, Jun Ogihara, Noriyuki Iwabuchi, Michio Sunairi

    Microbes and environments29 ( 4 ) 431 - 3   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We recently reported that the overexpression of GroEL2 played an important role in increasing the alkane tolerance of Rhodococcus erythropolis PR4. In the present study, we examined the effects of the introduction of groEL2 on the alkane tolerance of other Rhodococcus strains. The introduction of groEL2 into Rhodococcus strains led to increased alkane tolerance. The translocation of R. rhodochrous ATCC12674 cells to and survival in the n-octane (C8) phase in two phase culture were significantly enhanced by the introduction of groEL2 derived from strain PR4, suggesting that engineering cells to overexpress GroEL2 represents an effective strategy for enhancing organic solvent tolerance in Rhodococcus.

    DOI: 10.1264/jsme2.ME14114

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